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HS-(CH2)2-CONH-(CH2)4-NTA (FT 016)


SAMs terminated with a nitrilotriacetic acid (NTA-SAM) chelating agent bind Ni(II) and the His-Tag motif selectively and tightly. Consequently, they are ideal constructs for selective immobilization of His-tagged proteins (human TATA box binding protein, transcriptional activator Gal 4, two components of the yeast RNA polymerase II holoenzyme, and a single chain T-cell receptor construct) through complexation of the Ni(II) atom by two histidines. See: Sigal, G.B.; Bamdad, C.; Barberis, A.; Strominger, J.; Whitesides, G.M. Anal. Chem.199668, 490. This molecule utilise the OEG spacer responsible for its high accecibility on the distal surface of SAM combined with low non-specific binding background. For other option please compare out simple NTA presenting thiol HS-(CH2)11-(EG)3-NTA in the Thiols for Bioresistant and Biospecific SAMs cathegory.

Standard Products

name catalog no. m n weight price
HS-CH2-CONH-(CH2)4-NTA FT 016-m4-0.05 4 50 mg Log in to see the prices
HS-CH2-CONH-(CH2)4-NTA FT 016-m4-0.1 4 100 mg

Custom synthesis variants

Note, if none of the listed structures fit your specific requirements, please submit your own structure formula within our custom synthesis contact form by clicking button below.

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